Journal: Psychopharmacology

Article Title: Quantification of Ten Neuroactive Steroids in Plasma in Withdrawal Seizure–Prone and –Resistant Mice During Chronic Ethanol Withdrawal

doi: 10.1007/s00213-014-3618-y

Figure Lengend Snippet: Steroids that were simultaneously quantified in the GC-MS assay are indicated with a BOLD asterisk. Note that the potent positive modulators of GABAA receptors (THP, THDOC, androstanediol) are formed by the 2-step reduction of the parent steroids, progesterone, deoxycorticosterone, and testosterone, respectively. Also depicted are the sulfated derivatives of pregnenolone and DHEA (dehydroepiandrosterone), which are negative modulators of GABAA receptors. [DHDOC = dihydrodeoxycorticosterone; THDOC = tetrahydrodeoxycorticosterone; DHP = dihydroprogesterone; THP = tetrahydroprogesterone; DHT = dihydrotestosterone; HSD = hydroxysteroid dehydrogenase]

Article Snippet: This finding is consistent with the reported decrease in plasma testosterone levels in male alcoholics (e.g., Castilla-Garcia et al., 1987; Iturriaga et al., 1999 ; Krüger et al, 2006 ; Maneesh et al., 2006 ; Martinez-Riera et al., 1995 ; Ruusa et al., 1997 ; Sierksma et al., 2004 ; Ylikahri et al., 1980 ).

Techniques: Gas Chromatography-Mass Spectrometry

Journal: Psychopharmacology

Article Title: Quantification of Ten Neuroactive Steroids in Plasma in Withdrawal Seizure–Prone and –Resistant Mice During Chronic Ethanol Withdrawal

doi: 10.1007/s00213-014-3618-y

Figure Lengend Snippet: TIC was obtained for a reference standard mixture (1000 pg of each steroid) using selected ion monitoring (SIM) in negative chemical ionization mode. There was high response and good resolution for all analytes of interest; x-axis depicts range of retention times (min) for elution of the neurosteroid groups (also see Table 1). [Testosterone derivatives = 3α,5α-A-Diol & 3α,5β-A-Diol (androstanediols); Androstenedione derivatives = 3α,5α-A & 3α,5β-A (hydroxyandrostan-17-ones); Progesterone derivatives = 3α,5α-THP (allopregnanolone) & 3α,5β-THP (pregnanolone); Deoxycorticosterone derivatives = 3α,5α-THDOC & 3α,5β-THDOC (tetrahydrodexoycorticosterones)] See Figure 1 for parent steroids.

Article Snippet: This finding is consistent with the reported decrease in plasma testosterone levels in male alcoholics (e.g., Castilla-Garcia et al., 1987; Iturriaga et al., 1999 ; Krüger et al, 2006 ; Maneesh et al., 2006 ; Martinez-Riera et al., 1995 ; Ruusa et al., 1997 ; Sierksma et al., 2004 ; Ylikahri et al., 1980 ).

Techniques: